Products

Regis Technologies Chiral Columns

Protein-based Chiral Columns

The Analytical Solution for an Extremely Broad Range of Compounds
Regis carries a line of protein-based chiral columns manufactured by ChromTech including the Chiral AGP (α-glycoprotein), Chiral CBH (cellobiohydrolase), and Chiral HSA (human serum albumin), in micro, analytical and semi-preparative sized columns.

Chiral AGP

A chiral HPLC-column for direct resolution of enantiomers
The chiral selector in the CHIRAL-AGP phase is alpha1-acid glycoprotein (AGP). This very stable protein has been immobilized onto spherical 5 micrometer silica particles. The protein tolerates high concentrations of organic solvents, high and low pH, and high temperatures. The column is used in the reversed-phase and can be used for direct resolution of enantiomers, without derivatization. The CHIRAL-AGP column separates enantiomers of an extremely broad range of drug compounds:
- amines (primary, secondary, tertiary, and quaternary)
- acids (strong and weak)
- nonprotolytes (amides, esters, alcohols, sulphoxides, etc.)

Spherical Silica

Phase Code Particle Size Column Dimensions Part Number Add
Chiral AGP 5 µm 1 cm x 3.0 mm i.d. guard 732300
Chiral AGP 5 µm 10 cm x 10.0 mm i.d. 732301
Chiral AGP 5 µm 10 cm x 2.0 mm i.d. 732196
Chiral AGP 5 µm 10 cm x 4.0 mm i.d. 732200
Chiral AGP 5 µm 15 cm x 10.0 mm i.d. 732302
Chiral AGP 5 µm 15 cm x 2.0 mm i.d. 732197
Chiral AGP 5 µm 15 cm x 4.0 mm i.d. 732199
Chiral AGP 5 µm 5 cm x 2.0 mm i.d. 732201
Chiral AGP 5 µm 5 cm x 3.0 mm i.d. 732203
Chiral AGP 5 µm 5 cm x 4.0 mm i.d. 732198

Chiral CBH

A chiral HPLC-column for direct resolution of enantiomers
Cellobiohydrolase (CBH) is a very stable enzyme, which has been immobilized onto 5 micrometer spherical silica particles. This is a reversed-phase column, used for the direct separation of enantiomers. The column is preferantially used for the separation of enantiomers of basic drugs from many compound classes. CHIRAL-CBH separates preferentially compounds containing one or more basic nitrogen together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.).

Spherical Silica

Phase Code Particle Size Column Dimensions Part Number Add
Chiral CBH 5 µm 1 cm x 3.0 mm i.d. guard 732358
Chiral CBH 5 µm 10 cm x 10.0 mm i.d. 732355
Chiral CBH 5 µm 10 cm x 2.0 mm i.d. 732353
Chiral CBH 5 µm 10 cm x 4.0 mm i.d. 732350
Chiral CBH 5 µm 15 cm x 10.0 mm i.d. 732356
Chiral CBH 5 µm 15 cm x 2.0 mm i.d. 732354
Chiral CBH 5 µm 15 cm x 4.0 mm i.d. 732351
Chiral CBH 5 µm 5 cm x 4.0 mm i.d. 732352

Chiral HSA

A chiral HPLC-column for direct resolution of enantiomers
The CHIRAL-HSA column is based on human serum albumin (HSA). The protein has been immobilized onto spherical 5 micrometer particles. Enantiomers of preferentially acidic compounds can be resolved directly, without derivatization. CHIRAL-HSA is especially suited for the resolution of weak and strong acids, zwitterionic and nonprotolytic compounds. However, basic compounds have also been resolved.

Spherical Silica

Phase Code Particle Size Column Dimensions Part Number Add
Chiral HSA 5 µm 1 cm x 3.0 mm i.d. guard 732340
Chiral HSA 5 µm 10 cm x 10.0 mm i.d. 732341
Chiral HSA 5 µm 10 cm x 2.0 mm i.d. 732202
Chiral HSA 5 µm 10 cm x 4.0 mm i.d. 732240
Chiral HSA 5 µm 15 cm x 10.0 mm i.d. 732342
Chiral HSA 5 µm 15 cm x 2.0 mm i.d. 732238
Chiral HSA 5 µm 15 cm x 4.0 mm i.d. 732239
Chiral HSA 5 µm 5 cm x 2.0 mm i.d. 732243
Chiral HSA 5 µm 5 cm x 3.0 mm i.d. 732242
Chiral HSA 5 µm 5 cm x 4.0 mm i.d. 732241



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