ES Notes October 2011

In this issue of ESNotes we would like to share with you some of the exciting recent product introductions and enhancements including some from past ES Notes:

• The new GreenSep Nitro SFC stationary phase which has proven superior to "older" normal phase materials,
• Epic HILIC-HC sub-2um Columns for high sample loadings for trace analysis and
• The final part of Preparative HPLC Practical Consideration a presentation outlining the essential elements in developing a plan for scaling up to the preparative HPLC separation.

All designed to be technically informative and practically useful.

Epic HILIC-HC sub-2um HPLC Columns

Epic HILIC-HC (HC for high capacity) is a new stationary phase for HILIC chromatography. Epic HILIC-HC is specifically designed for HILIC chromatography and can achieve high performance separations, yield rugged methods and deliver long column life times. To extend the performance of Epic HILIC-HC we are pleased to introduce Epic HILIC-HC 1.8μm columns for use with ultra high pressure liquid chromatographs. These columns have been engineered specifically for chromatography at pressures up to 19000 psi. The Epic 1.8μm columns will provide the chromatographer with the highest resolution over a wide variety of flow rate conditions and mobile phase compositions. The linked chromatogram highlights of the unique capability for Epic HILIC-HC, in this chromatogram toluene is less retained than uracil. Uracil has been traditionally used as an unretained marker for the determination of void volume, however with Epic HILIC-HC and an 80% acetonitrile mobile phase uracil can be retained.

Click on the link for example applications. More ....

GreenSep™ Nitro SFC Columns

GreenSep™ Nitro SFC a new stationary phase for SFC has proven superior to conventional stationary phases (such as diol, cyano etc...) in the areas of separation selectivity and loading capacity. The linked chromatogram is a prime example of the separation selectivity obtainable with the GreenSep Nitro SFC column. GreenSep Nitro SFC is specifically designed for the separation of geometrical isomers as well as diastereomers. It is the column of choice in separating compounds that contain an aromatic group, polarizable electrons, halogenated groups and conjugate systems. GreenSep Nitro SFC is unsurpassed for its separation capabilities and is available in analytical columns, preparative columns and bulk media.

Click on the link for Part 1. More ....

Preparative HPLC - Practical Considerations Part 2.

The use of an analytical column is the key step in developing any preparative HPLC separation. In order to develop and optimize a preparative HPLC separation a variety of analytical columns should be evaluated. The analytical column is essential in evaluating the chromatographic separation and developing a plan for scaling up to the preparative HPLC separation.In the first part of this presentation several important factors were discussed including column length, column diameter and column flow. In this second part of the presentation we will discuss particle size, stationary selection and mobile phase selection.

Click on the link for Part 1. More ....